Numerical Chromosomal Aberrations In Renal Tumor Detected By Fluorescence In Situ Hybridization.
Y. Wada*, H. Yokogi, N. Moriyama-Gonda, M. Igawa, and T. Ishibe Dept. of Urology, Shimane Medical University, Japan.
In renal tumors, the correlation between numerical chromosomal aberrations and the histopathological findings is unknown. Touch smear samples of renal tumors were assessed by the fluorescence in situ hybridization tequnique, using chromosome 3, 7, 9, and 17 specific centromere DNA probes. Monosomy of chromosomes 3 was observed in thirteen of the 14 RCCs and one oncocytoma. All of 6 diploid RCCs and seven of 8 aneuploid RCCs demonstrated monosomy 3, while polysomy 3 was shown in four of 8 aneuploid RCCs. Moreover polysomy 7 was detected in all aneuploid cases. In respective chromosomes, aneuploid tumors had more polisomy rates than diploid tumors. To investigate the relationship between chromosomal aberrations and histopathological factors described in RCC, we assessed the rate of hyperdiploid cells (three or more spots) for same chromosomes described above. It was highly correlated with increasing tumor grade, increasing pathological stage, growth patterns of tumors, and venous involvement. The correlation between the chromosomal aberrations and histopathological findings in our study may indicate the numerical chromosomal aberrations to be important indicator of the malignant potential of RCCs.